Plant Hormones

Produktbeschreibung

This volume aims to present a representative cross-section of modern experimental approaches relevant to Plant Hormone Biology, ranging from relatively simple physiological to highly sophisticated methods. Chapters describe physiological, developmental, microscopy-based techniques, measure hormone contents, and heterologous systems. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Plant Hormones:Methods and Protocols, Third Edition aims to provide researchers with useful methods to advance their research. 

Inhaltsverzeichnis

Real Time Analysis of the Apical Hook Development.-ÿGrafting with Arabidopsis thaliana.-ÿTips and Tricks for Exogenous Application of Synthetic Post-Translationally Modified Peptides to Plants.-ÿAssaying Germination and Seedling Responses of Arabidopsis to Karrikins.-ÿLow-cost Microprocessor-Controlled Rotating Stage for Medium-Throughput Time-Lapse Plant Phenotyping.-ÿGenome-Wide Association Mapping of Root Traits in the Context of Plant Hormone Research.-ÿHigh throughput scoring of Seed Germination.-ÿHistochemical Staining of beta-Glucuronidase and its Spatial Quantification.-ÿImaging TCSn::GFP, A Synthetic Cytokinin Reporter, in Arabidopsis thaliana.-ÿHighlighting Gibberellins Accumulation Sites in Arabidopsis Thaliana Root Using Fluorescently Labeled Gibberellins.-ÿIn-silico Methods for Cell Annotation, Quantification of Gene Expression and Cell Geometry at Single Cell Resolution using 3DCellAtlas.-ÿAnalysing Cell Wall Elasticity after Hormone Treatment: An example using Tobacco BY-2 Cells and Auzin.-ÿFRET-FLIM for Visualizing and Quantifying Protein Interactions in Live Plant Cells.-ÿIn vivo Identification of Plant Protein Complexes using IP-MS/MS.-ÿAssaying auxin Receptor Activity using SPR assays with F-Box Proteins and Aux-IAA degrons.-ÿStudying Transcription Factor Binding To Specific Genomic Loci by Chromatin Immunoprecipitation (ChIP).-ÿHormone Receptor Glycosylation.-ÿHighly Sensitive Salicylic Acid Quantification in Milligram Amounts of Plant Tissue.-ÿHigh-Resolution Cell-Type Specific Analysis of Cytokinins in Sorted Root Cell Populations of Arabidopsis thaliana.-ÿHormone Profiling in Plant Tissues.-ÿUse of Xenopus laevis Oocytes to Study Auxin Transport.-ÿCharacterizing Auxin Response Circuits in Saccharomyces cerevisiae by Flow Cytometry.ÿ