Analgesia

Produktbeschreibung

Chronic pain is a complex phenomenon, which continues to remain undertreated in the majority of affected patients thus representing a significant unmet medical need, but the development of cellular, subcellular, and molecular methods of approaching this epidemic of pain shows great promise. In Analgesia: Methods and Protocols, experts in the field present thorough coverage of molecular analgesia research methods from target discovery through target validation and clinical testing to tolerance and dependence, with extensive chapters on emerging receptor classes as targets for analgesic drugs and innovative analgesic strategies. As a volume in the highly successful Methods in Molecular Biology¿ series, the chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes sections with tips on troubleshooting and avoiding known pitfalls. Comprehensive and essential, Analgesia: Methods and Protocols promises to aid and enrich the research of all those scientists and clinicians who are interested in what the increasingly molecular future has in store for analgesia research, from the molecular research bench through the animal laboratory to the bedside. 

Inhaltsverzeichnis

Alternatives to Mammalian Pain Models 1: Use of C. elegans for the Study of
Volatile Anesthetics.- Alternatives to Mammalian Pain Models 2: Using Drosophila
to Identify Novel Genes Involved in Nociception.- Animal Models of Acute
Surgical Pain.- Animal Models of Acute and Chronic Inflammatory and Nociceptive
Pain.- Noxious Heat Threshold Measured with Slowly Increasing Temperatures:
Novel Rat Thermal Hyperalgesia Models.- Locomotor Activity in a Novel
Environment as a Test of Inflammatory Pain in Rats.- Rationale and Methods for
Assessment of Pain-Depressed Behavior in Preclinical Assays of Pain and
Analgesia.- Animal Models of Orofacial Pain.- Migraine Models.- Experimental
Models of Visceral Pain.- Human Correlates of Animal Models of Chronic Pain.-
Human Experimental Pain Models 1: The Ultraviolet Light UV-B Pain Model.- Human
Experimental Pain Models 2: The Cold Pressor Model.- Human Experimental Pain
Models 3: Heat/Capsaicin Sensitization and Intradermal Capsaicin Models.- The
Value of the Dental Impaction Pain Model in Drug Development.- Live Cell Imaging
for Studying G Protein-Coupled Receptor Activation in Single Cells.- Recombinant
Cell Lines Stably Expressing Functional Ion Channels.- Ion Channels in Analgesia
Research.- Electrophysiological and Neurochemical Techniques to Investigate
Sensory Neurons in Analgesia Research.- The Genetics of Pain and Analgesia in
Laboratory Animals.- RT-PCR Analysis of Pain Genes: Use of Gel-Based RT-PCR for
Studying Induced and Tissue-Enriched Gene Expression.- Gene-Based Approaches in
the Study of Pathological Pain.- Linkage Analysis and Functional Evaluation of
Inherited Clinical Pain Conditions.- Rat Bone Marrow Stromal Cells and
Oligonucleotides in Pain Research.- Transplantation of Human Mesenchymal Stem
Cells in the Studyof Neuropathic Pain.- Delivery of RNA Interference to
Peripheral Neurons In Vivo Using Herpes Simplex Virus.- Combination of Cell
Culture Assays and Knockout Mouse Analyses for the Study of Opioid Partial
Agonism.- Assessing Potential Functionality of Catechol-O-methyltransferase
(COMT) Polymorphisms Associated with Pain Sensitivity and Temporomandibular
Joint Disorders.- Genetic Polymorphisms and Human Sensitivity to Opioid
Analgesics.- Molecular Assays for Characterization of Alternatively Spliced
Isoforms of the Mu Opioid Receptor (MOR).- Inhalational Anesthetic
Photolabeling.- Measuring Membrane Protein Interactions Using Optical
Biosensors.- Proteomics and Metabolomics and Their Application to Analgesia
Research.- Preemptive Analgesia: Problems with Assessment of Clinical
Significance.- Standardization of Pain Measurements in Clinical Trials.-
Procedural Sedation and Analgesia Research.- Non-invasive Transcranial Direct
Current Stimulation for the Study and Treatment of Neuropathic Pain.- Pain
Imaging in the Emerging Era of Molecular Medicine.- Current and Emerging
Pharmacologic Therapies for Pain and Challenges Which Still Lay Ahead. 

Kritik

From the reviews:

"The text provides all one wishes to know about research methods in analgesia. ... editor's decision to invite groups of authors and experts from the different areas of research in analgesia, has allowed a broad and up-to-date view of the field. ... This book is of great value for investigators, students and physicians interested in understanding pain and the mechanisms involved in its relief. On consulting this book, researchers, students and professionals will have the opportunity to update their knowledge of experimental methods in analgesia." (Zuleica Bruno Fortes, Brazilian Journal of Pharmaceutical Sciences, Vol. 46 (4), October-December, 2010)
"This book is a collection of methods used to study pain physiology, pharmacology, and genetics described by researchers in the field. ... intended to assist basic scientists and clinicians who are interested in the molecular aspects of pain research. ... This is a most unique book in the field of pain medicine. ... The book imparts a lot of information about the future of pain medicine and is a must read for pain physicians who want to be at their best when managing their patients." (Tariq M. Malik, Doody's Book Reviews, August, 2010)