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Plant Hormones

Methods and Protocols

von Springer-Verlag GmbH

This volume aims to present a representative cross-section of modern experimental approaches relevant to Plant Hormone Biology, ranging from relatively simple physiological to highly sophisticated methods. Chapters describe physiological, developmental, microscopy-based techniques, measure hormone contents, and heterologous systems. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Plant Hormones:Methods and Protocols, Third Edition aims to provide researchers with useful methods to advance their research.

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Produktbeschreibung

This volume aims to present a representative cross-section of modern experimental approaches relevant to Plant Hormone Biology, ranging from relatively simple physiological to highly sophisticated methods. Chapters describe physiological, developmental, microscopy-based techniques, measure hormone contents, and heterologous systems. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Plant Hormones:Methods and Protocols, Third Edition aims to provide researchers with useful methods to advance their research. 

Inhaltsverzeichnis

Real Time Analysis of the Apical Hook Development.- Grafting with Arabidopsis thaliana.- Tips and Tricks for Exogenous Application of Synthetic Post-Translationally Modified Peptides to Plants.- Assaying Germination and Seedling Responses of Arabidopsis to Karrikins.- Low-cost Microprocessor-Controlled Rotating Stage for Medium-Throughput Time-Lapse Plant Phenotyping.- Genome-Wide Association Mapping of Root Traits in the Context of Plant Hormone Research.- High throughput scoring of Seed Germination.- Histochemical Staining of beta-Glucuronidase and its Spatial Quantification.- Imaging TCSn::GFP , A Synthetic Cytokinin Reporter, in Arabidopsis thaliana.- Highlighting Gibberellins Accumulation Sites in Arabidopsis Thaliana Root Using Fluorescently Labeled Gibberellins.- In-silico Methods for Cell Annotation, Quantification of Gene Expression and Cell Geometry at Single Cell Resolution using 3DCellAtlas.- Analysing Cell Wall Elasticity after Hormone Treatment: An example using Tobacco BY-2 Cells and Auzin.- FRET-FLIM for Visualizing and Quantifying Protein Interactions in Live Plant Cells.- In vivo Identification of Plant Protein Complexes using IP-MS/MS.- Assaying auxin Receptor Activity using SPR assays with F-Box Proteins and Aux-IAA degrons.- Studying Transcription Factor Binding To Specific Genomic Loci by Chromatin Immunoprecipitation (ChIP).- Hormone Receptor Glycosylation.- Highly Sensitive Salicylic Acid Quantification in Milligram Amounts of Plant Tissue.- High-Resolution Cell-Type Specific Analysis of Cytokinins in Sorted Root Cell Populations of Arabidopsis thaliana.- Hormone Profiling in Plant Tissues.- Use of Xenopus laevis Oocytes to Study Auxin Transport.- Characterizing Auxin Response Circuits in Saccharomyces cerevisiae by Flow Cytometry. 

Mehr vom Verlag:

Springer-Verlag GmbH

Mehr aus der Reihe:

Methods in Molecular Biology

Produktdetails

Medium: Buch
Format: Gebunden
Seiten: xi, 288
Sprache: Englisch
Erschienen: November 2016
Auflage: 3rd edition
Band-Nr.: Band 1
Sonstiges: .978-1-4939-6467-3
Maße: 260 x 183 mm
Gewicht: 765 g
ISBN-10: 1493964674
ISBN-13: 9781493964673
Verlagsbestell-Nr.: 978-1-4939-6467-3

Bestell-Nr.: 17607894 
Libri-Verkaufsrang (LVR):
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Bestell-Nr. Verlag: 978-1-4939-6467-3

LIBRI: 8362815
LIBRI-EK*: 52.22 € (25.00%)
LIBRI-VK: 74,50 €
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KNO: 58189175
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KNO-SAMMLUNG: Methods in Molecular Biology 1497
P_ABB: Bibliographie
KNOABBVERMERK: 3. Aufl. 2016. xi, 288 S. 11 SW-Abb., 106 Farbabb. 254 mm
KNOSONSTTEXT: .978-1-4939-6467-3
KNOMITARBEITER: Herausgegeben:Kleine-Vehn, Jürgen; Sauer, Michael
KNO-BandNr. Text:Band 1
Einband: Gebunden
Auflage: 3rd edition
Sprache: Englisch
Beilage(n): Book

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